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May 2018

Confocal Raman Microscopy 2nd Edition – At bookstores now

Confocal Raman Microscopy
Editors: Jan Toporski, Thomas Dieing, Olaf Hollricher
ISBN: 978-3-319-75380-5

Confocal Raman Microscopy, edited by members of the WITec team and part of the Springer Series in Surface Sciences, has been thoroughly revised and expanded for its 2nd Edition. The updated book is available now and features the latest developments in Raman techniques and instrumentation.

As with the 1st Edition, which has already established itself as the preeminent resource for the technique in labs and libraries around the world, the new edition provides a comprehensive overview of the fundamentals, practical considerations and real-world applications of Raman microscopy. Additionally, it has nearly doubled in number of chapters and now includes sub-sections on theory and technology, novel materials, geosciences, life and pharmaceutical sciences, materials science and a historical overview of the Raman effect. It also explores the rapidly evolving field of correlative microscopy by detailing how 3D Raman imaging can be integrated with other investigative methods to achieve a far greater understanding of a sample’s properties.

The 2nd Edition of Confocal Raman Microscopy, edited by WITec scientists Dr. Olaf Hollricher and Dr. Jan Toporski, can be purchased in print or e-book formats directly from Springer or through online shops. Order your copy today and have the current state of the art in Confocal Raman Microscopy at your fingertips.

March 2018

alpha300 Ri - New inverted confocal Raman microscope

WITec’s proven 3D Raman imaging functionality is now available in an inverted microscope.

WITec has launched the alpha300 Ri inverted Raman microscope. It combines the advantages of data acquisition from below with the established merits of 3D confocal Raman imaging, a powerful and versatile technique that can chemically characterize samples nondestructively and without labeling or other specialized preparation. The speed, sensitivity and resolution of the WITec alpha300 series are now available from a new angle. Research applications in the fields of life sciences, biomedicine and pharmaceutics will benefit in particular from the new setup.

The inverted beam geometry of the alpha300 Ri delivers many advantages in sample access and handling. Specimens in aqueous environments such as cell cultures can be examined more effectively. Standardized liquid sample holder formats can be quickly and easily mounted and measured. This accelerates the experimental workflow and helps ensure consistency. Investigations in materials science will be aided by the very large working area that can accommodate bulky samples and the set focal plane. The motorized sample stage also facilitates the mounting of environmental enclosures and other accessories.

Many modular components and upgrade possibilities developed for the WITec alpha300 series are compatible with the Ri version. Other microscopy techniques associated with inverted microscopes, such as fluorescence, differential interference contrast (DIC) and phase-contrast can also be easily integrated.

According to Olaf Hollricher, Director of Research and Development at WITec, “Researchers in life sciences are accustomed to working with inverted microscopes and their advantages for in vivo measurements, among others, are well-established. Developing a variant of our alpha300 confocal Raman imaging series with an inverted beam is a logical step in the evolution of our product line. Now the benefits of nondestructive, label-free molecular characterization are available from below the sample plane, backed up by all the modularity and upgradeability inherent in our systems.”

The WITec alpha300 Ri will be showcased at Analytica 2018 in Munich at the WITec booth A2/402. For more information, please visit the product webpage >>>.

March 2018

WITec Releases TrueMatch - Integrated Raman Spectral Database Software

TrueMatch is a powerful and innovative software component for accessing and developing Raman spectral databases

WITec, manufacturer of 3D Raman imaging systems, is now offering TrueMatch, a powerful software component for accessing and creating Raman spectral databases. It can search existing libraries of Raman spectra to identify sample components and also allows users to create their own catalog of acquired spectra to assist in their research.

TrueMatch is available as a fully integrated option with the WITec Project FIVE software environment and databases can be brought directly into use in post-processing. Multi-spectral searches enable the user to identify several spectra simultaneously, and return hit rates that show the probability of a match for each individual spectrum. This greatly accelerates the process of characterization and is a perfect complement to WITec’s standard data analysis features. Demix search uses databases to describe acquired spectra in terms of pure substances, which can then be physically located in an image. Seeing the distribution of pure chemical components matched to sample features gives the researcher a precise visualization. Generated reports can be stored for further use in subsequent measurements, amplifying the effectiveness of the system as a whole and turning meaningful results into a valuable resource.

In addition to the proprietary WITec Raman database capability included with TrueMatch, compatible versions of ST Japan’s wide range of databases are available. Extensive libraries of Raman spectra can be referenced while new ones are created, all from within WITec’s fully-integrated software and hardware environment.

“TrueMatch makes it easier for the user to interpret their Raman images. It also greatly simplifies the process of searching through Raman spectral databases and creating new ones based on user-generated data compared to anything else out there. It’s the perfect complement to the standard analysis methods WITec implements in our Suite FIVE software environment,” says Olaf Hollricher, Managing Director and Director of Development at WITec, in summarizing the advantages of the new software.

Visit the TrueMatch product page for detailed information on the new software.

 

December 2017

WITec Suite FIVE Software Now Features Active Focus Stabilization

3D Raman image of cellulose fibers and additives in paper. In order to document the precision of the new focus stabilization, a very long measurement time of 46 hours was chosen.

WITec, industry-leading manufacturer of Raman imaging and correlative microscopy systems, has started shipping its Suite FIVE data acquisition, evaluation and processing software with the new ability to compensate for thermal and mechanical variations during long-term measurements.

Confocal Raman microscopy investigations performed on delicate samples often require lower excitation power and a corresponding increase in acquisition time. Active focus stabilization ensures that the ideal focus between the microscope objective and sample is maintained and signal collection is unaffected while temperature or humidity changes. This is accomplished by an automatic routine that uses a reference point to optimize the Raman signal.

This most recent development in WITec’s ongoing effort to provide results-oriented functionality is a standard and fully-integrated enhancement to the powerful Suite FIVE software package.

October 2017

Notes on the 14th Confocal Raman Imaging Symposium

Winners of the Poster Award Thomas Rabl (left) and Vera Dugandžic with WITec’s CEO Joachim Koenen (right).

In the shadow of the world's tallest church, more than 100 scientists met in Ulm at the end of September for the 14th Confocal Raman Imaging Symposium. The lectures thoroughly documented how modern Raman microscopy contributes to gaining new insights into widely varied scientific questions.

In 11 invited lectures three short presentations, the speakers presented new and interesting applications of Raman microscopy to answer questions from life sciences, materials science, geoscience and environmental science.

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